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bio rad mini protean ii electroblotting apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad bio rad mini protean ii electroblotting apparatus
    Bio Rad Mini Protean Ii Electroblotting Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 30494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+mini+protean+ii+electroblotting+apparatus/Nitrocellulose+Membranes/10__1128_slash_jvi__00720___17-76-34-34
    Average 99 stars, based on 30494 article reviews
    bio rad mini protean ii electroblotting apparatus - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: The Polypyrimidine Tract-Binding Protein Affects Coronavirus RNA Accumulation Levels and Relocalizes Viral RNAs to Novel Cytoplasmic Domains Different from Replication-Transcription Sites
    Article Snippet: Cell lysates were analyzed by denaturing electrophoresis in NuPAGE 4 to 12% bis-Tris gels with MOPS-SDS running buffer (Invitrogen). .. Proteins were transferred to a nitrocellulose membrane (Hybond-C extra nitrocellulose; Amersham Biosciences) with a Bio-Rad Mini protean II electroblotting apparatus at 100 V for 1 h in bis-Tris transfer buffer (25 mM bis-Tris, 25 mM bicine, 1 mM EDTA) containing 20% methanol. .. Membranes were blocked for 1 h with 5% dried skim milk in Tris-buffered saline (20 mM Tris-HCl, pH 7.5, 150 mM NaCl) and then probed with antibodies specific for PTB (mouse monoclonal antibody [MAb] from hybridoma BB7; ATCC), TGEV nucleoprotein (N; mouse MAb 3DC10) (48), and -actin (mouse MAb ab8226; Abcam).

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.

    Nucleic Acid Electrophoresis:

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.

    Western Blot:

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.

    Transfection:

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.

    Electrophoresis:

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.

    Saline:

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.

    Incubation:

    Article Title: Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A Virus
    Article Snippet: .. 235 Protein gel electrophoresis and Western blot analysis 236 Proteins from transfected cell lysates were separated by denaturing electrophoresis 237 using 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-238 Rad) with a Bio-Rad Mini Protean II electroblotting apparatus at 100 V for 2 h. 239 Membranes were blocked for 1 h with 5% dried skim milk in phosphate-buffered saline 240 (PBS) containing 0.1% Tween 20 (T-PBS) and incubated overnight at 4°C with primary 241 rabbit or goat polyclonal antibodies (pAbs) against NS1 (55), PA (ABIN398948; 242 antibodies-online), NP (55), PB2 (SC17603; Santa Cruz Biotechnology), PB1 243 (SC117601; Santa Cruz Biotechnology),or a mouse monoclonal antibody (mAb) against 244 NP (ATCC HB-65). .. Mouse mAb against actin (A1978; Sigma) was used as an internal 245 loading control.



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    Bio-Rad bio rad mini protean ii electroblotting apparatus
    Bio Rad Mini Protean Ii Electroblotting Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+mini+protean+ii+electroblotting+apparatus/Nitrocellulose+Membranes/10__1128_slash_jvi__00720___17-76-34-34
    Average 99 stars, based on 1 article reviews
    bio rad mini protean ii electroblotting apparatus - by Bioz Stars, 2026-09
    99/100 stars
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